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Study on optimizing the preparation conditions and characterizing of novel nylon affinity membranes
Authors： NIE Huali12, Chen Tianxiang2, ZHU Limin2
Units： 1. Key Laboratory of Science & Technology of Eco-Textil, Ministry of Education, Donghua University Shanghai 201620, China; 2. College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China
KeyWords： Affinity membrane chromatography；chitosan；papain；cystatin
ClassificationCode：TQ028.8
year,volume(issue):pagination： 2011, 31(2):74-77

Abstract：

 Nylon membranes are first hydrolyed with dilute HCl and then treated with chitosan  before being used as the affinity carrier. Papain as a ligand has been immobilized on the actived membranes with glutaraldehyde as crosslinking agent. The factors involving with the activity of immobilized papain, such as concentration of glutaraldehyde, pH, temperature, reaction time, and the amount of added papain have been studied. The results show that the optimum conditions for papain immobilization are as follows: pH 9.0, 0.5% glutaraldehyde solution, the concentration of added papain is 10 mg/mL, the reaction time is 6 h at 45℃. The nylon membrane prepared is then used to purify cystatin from potato juice, and showed that they are high efficiency affinity membranes base for cystatin separation.

Funds：
国家自然科学基金（50773009）；教育部高校博士点新教师基金（20090075120001）；中央高校基本科研业务费专项基金

AuthorIntro：
聂华丽（1978-）, 女，上海市人，博士，讲师，从事蛋白质分离与纯化研究，电话：021-67792659，E-Mail：niehuali2004@126.com

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