Membrane Science and Technology

Position：Home >> Abstract

Endotoxin removal from human plasma through PVDF affinity membrane containing Ser ligand

Authors： Kun Xu1, Man Huang3, Liguang Wu2, Lin Zhang1, Huanlin Chen1

Units： 1. Department of Chemical & Biochemical Engineering, Zhejiang University, Engineering Research Center of Membrane and Water Treatment Technology, MOE, Hangzhou, 310027, China2. College of Environ. Sci. & Eng., Zhejiang Gongshang University, Hangzhou, 310035, China;3. Sir Run Run Shaw Hospital, Zhejiang University, 310016, China

KeyWords： endotoxin; affinity membrane; PVDF; Ser; human plasma

ClassificationCode：TQ028.8

year,volume(issue):pagination： 2011, 31(2):28-34

Abstract：

A new affinity membrane, PVDF hollow fiber membrane with L-serine (Ser) ligand was prepared (with 1,6-hexanediamine as the space arm) for endotoxin removal from human plasma. FTIR and X-ray photoelectron spectroscopy (XPS) were used to analyse the surface of membranes. The effects of operation parameters，such as pH value and ionic strength，on the endotoxin adsorption capacity of the affinity membranes were investigated, so as to the affinity adsorption kinetics. Then, self-made affinity membrane module was used to remove endotoxin form human plasma., and the endotoxin adsorption capability is 0.029EU/cm2. For the human plasma sample with endotoxin concentration of 0.42EU/ml, the clearance efficiency of self-made module could reach 43.8% with little effect on other biochemical indicators.

Funds：

浙江省科技厅科技计划项目（2007C33008）；中央高校基本科研业务费专项资金资助（教育部膜与水处理技术工程研究中心, 浙江大学，KYJD09011）

AuthorIntro：

徐堃（1983-），男，山东烟台人，硕士研究生，主要从事亲和膜脱除内毒素研究. *通讯联系人

Reference：

[1] Hirayama C, Sakata M. Chromatographic removal of endotoxin from protein solutions by polymer particles [J]. Chromatogr B, 2002, 781: 419–32.
[2] Dainippon pharmaceuticals. An agent for removing nucleic acids or endotoxin and a method for the removal [P]. Eur, Patent, 0,240,348. 1987.
[3] Kurita water ind. removing nucleic acid and endotoxin from solution using chitosan treatment [P]. Jpn, Patent 07,289,238. 1995.
[4] Anspach FB, Hilbeck O. Removal of endotoxins by affinity sorbents [J]. Chromatogr A, 1995, 711: 81-92.
[5] Liu S, Tobias R, et al. Removal of endotoxin from recombinant protein preparations [J]. Clin Biochem 1997, 30: 455–63.
[6] Anspach FB, Petsch D. Membrane adsorbers for selective endotoxin removal from protein solutions [J]. Process Biochem, 2000, 35: 1005–12.
[7] Petsch D, Beeskow TC, Anspach FB, et al. Membrane adsorbers for selective removal of bacterial endotoxin [J]. Chromatogr B, 1997, 693: 79–91.
[8] Petsch D, Deckwer WD, Anspach FB, et al. Endotoxin removal with poly(ethyleneimine)-immobilized adsorbers: sepharose 4B versus flat sheet and hollow fibre membranes [J]. Chromatogr B, 1998, 707:121–30.
[9] Hanora A, Plieva FM, Hedstro¨m M, et al. Capture of bacterial endotoxins using a supermacroporous monolithic matrix with immobilized polyethyleneimine, lysozyme or polymyxin B [J]. Biotechnol, 2005, 118: 21–33.
[10] Acconci C, Legallais C, Vijayalakshmi MA, et al. Depyrogenation of snake antivenom serum [J]. Membr Sci , 2000, 173: 235–45.
[11] D.K. Roper, E.N. Lightfoot. Separation of biomolecules using adsorptive membranes [J]. Chromatogr A, 1995, 702: 3-26.
[12] J. Thommes, M.R. Kula. Membrane Chromatography-An Integrative Concept in the Downstream Processing of Proteins [J]. Biotechnol Prog, 1995, 11: 357-367.
[13] Zhong Wei, Wei Huang, Jihong Li, et al. Studies on endotoxin removal mechanism of adsorbents with amino acid ligands [J]. Journal of Chromatography B, 2007, 852: 288–292.
[14] Haixiang Sun, Lin Zhang, Hong Chai, Study of human -globulin adsorption capacity of PVDF hollow fiber affinity membranes containing different amino acid ligands [J]. Separation and Purification Technology, 2006, 48 : 215-222.
[15] S.H. Lee, J.S. Kim, C.W. Kim. Optimization of buffer conditions for the removal of endotoxins using Q-sepharose [J]. Process Biochemistry, 2003, 38: 1091-1098.
[16] Van Lenten, BJ,Fogelman, AM,Haberland, et al. The role oflipoproteins and receptor-mediated endocytosis in the transport of bacterial lipopolysaccharide [J]. Proc Natl Acad Sci USA. 1986, 83(8): 2704-2708.
[17] Victorov AV,Medvdeve NV,Gladkaya EM,Morozkin AD,Podrez EA,Kosykh VA.Compositon and structure of lippolysaccharide-human plasma low density lipoprotein complex.Analytical ultracentrifugation, 31P-NMR, ESR and fluorescence spectroscopy studies [J]. Biochim.Biophys.Acta.,1989, 984(1): 119-127.
[18] 唐守万. 芳香氨基酸及内毒素吸附剂的制备与应用[D]: [博士论文]. 大连: 中国科学院大连化学物理研究所, 2007. 57-58.
[19] Meltem Y?lmaz, Gülay Bayramo lu, M. Yakup Ar?ca,Separation and purification of lysozyme by Reactive Green 19 immobilised membrane affinity chromatography [J]. Food Chemistry. 2005, 89: 11-18

Service：

【Download】【Collect】

《膜科学与技术》编辑部 Address: Bluestar building, 19 east beisanhuan road, chaoyang district, Beijing; 100029 Postal code; Telephone：010-80492417/010-80485372; Fax：010-80485372 ; Email：mkxyjs@163.com