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Selective Separation of Succinic Acid From Simulated Fermentation Broth by Nanofiltration
Authors： WU Hao, JIANG Min※, WEI Ping, GAO Chao,YANG Gao, LEI Dan
Units： State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering,
KeyWords： succinic acid, nanofiltration, selective separation, simulated fermentation broth
ClassificationCode：S216，TQ92
year,volume(issue):pagination： 2011, 31(5):46-51

Abstract：

Many impurities existed in succinic acid fermentation broth, such as by-product organic acids(formic acid, acetic acid), residual substrate(glucose) and divalent ion (Mg2+ , SO42-) , which was unfavorable to purification. Separation of succinic acid from simulated fermentation broth by nanofiltration membrane LNG-NF-016(MWCO 160Da) was studied, For this purpose, the effect of operating parameters such as pH in the feed solution, operation pressure, MgSO4 concentration, succinic acid concentration, glucose concentration, cross-flow rate and operation temperature on nanofiltration was investigated using an experimental design technique. The Selectivity of nanofiltration depended on the degree of dissociation of succinic acid, At pH about 3.0, undissociated succinic acid could be separated from glucose and MgSO4, succinic acid permeation of 98%, glucose and MgSO4 rejection more than 85%,.The Divalent succinate could be separated from acetate and formate at pH about 6.0, succinic acid rejection of 90%, succinic acid concentration about 121 g/L in retentate, formic acid and acetic acid concentrations below 5 g/L. Succinic acid was separated selectively from by-product organic acids, residual substrate and divalent ion in simulated fermentation broth by the same nanofiltration membrane.

Funds：
国家“973”计划项目 (2009CB724701)；江苏省高校自然科学基础研究资助项目(08KJB180003)；南京工业大学青年教师学术基金；江苏省“六大人才高峰” 资助项目(06-A-047)

AuthorIntro：
吴昊（1976-），男，讲师，南京工业大学，博士研究生，研究方向：发酵工程、生物分离工程*通讯作者：姜岷（1972-），男，教授，南京工业大学，博士，研究方向：发酵工程、酶工程

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