Membrane Science and Technology

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Characteristics and Immobilization Process of CS-Cu Enzymatic Membrane

Authors： Ge Zheng1, Cai Bangxiao1，2 *

Units： 1.Institute of Membrane Science and Engineering, College of Food and Biological Engineering, Zhejiang Gongshang University, Hangzhou 310035）

KeyWords： enzymatic membrane reactor; immobilized enzyme; chitosan-Cu complex; polyphenol oxidase; response surface

ClassificationCode：TQ028.8,R978.1

year,volume(issue):pagination： 2012, 32(1):64-70

Abstract：

Enzymatic membrane reactor(EMR) with immobilization of polyphenol oxidase(PPO) is paid more attention due to its isolation and biochemical characteristics. In this paper, a enzymatic membrane immobilized PPO was prepared based on the chelation of PPO and Cu2+ in chitosan(CS)-Cu complex(CCC) with CS membrane as the carrier. The response surface methodology was applied to investigate the influence of three factors of copper ion sequestration, QCu2+, PPO concentration, CE, pH in immobilization process on the vitality of PPO immobilized in CCC. The relative activity of PPO in the solution and immobilized in the membrane was determined respectively. The characteristics of CS-Cu–PPO membrane were discussed primarily. The results show that the optimal conditions for immobilization of PPO into the CCC were temperature 40℃, pH 7.67, QCu2+ 30.07 mg/mL, CE 2.12 mg/mL. Michaelis constant and the relative activity of PPO immobilized in CCC were larger than that of the solution. Enzyme half-decline period was 30 days, and the relative activity of PPO after repeated use of eight times reduced to 79% for CS-Cu–PPO membrane. This work offers the significantly basis for development of EMR with special function.

Funds：

国家科技支撑计划项目(2007BAD62B05), 国家农业部行业科技开发专项(nyhyzx07-21)

AuthorIntro：

葛振(1985-), 男, 山东人, 食品科学硕士研究生，研究方向：新型分离膜及其应用工程. *通讯联系人: 蔡邦肖, 教授. 13958002662, caibangxiao@163.com

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