PA6 nanofibers,the thickness of which is relatively uniform, were prepared by electrospinning in this paper.The influence of sampling method and sampling number on tensile test of nanofiber membrane was discussed in detail.The results show that dispersion degree of testing results is becoming smaller as the sampling numbers increase in a certain range.And once exceed a certain limit, dispersion degree will be no more obvious.As a result,it is perfect when sampling number is 8.Three sampling methods were used for tensile test of nanofiber membrane,and it turned out that the thickness of samples obtained by parallel sampling method was more uniform than the other two methods,and this method was the most reasonable for the results of tensile test.As a result, parallel sampling method is the most suitable for nanofiber membrane by electrospinning as a sampling method.