The separation of Cr(Ⅵ) from drinking water by anion-exchange membrane was investigated without supplying electrical potential based on Donnan dialysis principle. The results show that the separation efficiency of Cr(Ⅵ) by anion-exchange membrane can achieve 86.4% under the conditions of influent Cr(Ⅵ) of 1.0 mg/L ( 0.0192 mmol/L ), initial pH of the feed solution of 6.95, NaCl concentration of 0.1 mol/L in the counterion solution, flow rate of the feed and counterion solution of 2.5 mL/min, stirring speed of 500 r/min, and phase temperature of 25℃. Using Na2SO4 solution as the counterion solution, the separation efficiency of Cr(Ⅵ) reduces. Adjusting initial pH of the feed solution to 11.0, the separation efficiency of Cr(Ⅵ) by anion-exchange membrane decreases to 78.9%. Increasing stirring speed of the two solutions beside the membrane, Cr(Ⅵ) in the feed chamber is more easily transferred to the receiver chamber through anion-exchange membrane. When phase temperature increases from 10℃ to 40℃, the residual Cr(Ⅵ) concentration in the feed chamber decreases from 0.21 mg/L to 0.12 mg/L.