With the hollow fiber membrane modules from Tianjin MTIMO Membrane Technology Co., Ltd to construct MBR system, the own-designed and manufactured pilot test on biological Nitrogen and Phosphorus Removal by using Reverse A2O-MBR process to treat wastewater was studied. The research results show that the average effluent concentrations of COD，NH3-N，TN and TP are 22.23mg/L,1.89mg/L,14.96mg/L,and 0.38mg/L respectively. The average removal rate of COD，NH3-N，TN and TP are 91.49%,94.5%，63.4%,and 87.3% respectively. The NTU of effluent was less than 0.19. Steadily operating data shows that the average effluent concentrations are achieving I-A criteria specified in the Discharge standard of pollutants for municipal wastewater treatment plant (GB18918-2002), and normally the average effluent concentrations are achieving the I-B criteria. The contaminate membrane modules were cleaned by the composite alkali substances or the composite alkali-acid substances.TMP(Trans-membrane pressure）are from 43kpa,73kpa falling to 12kpa,25kpa respectively. The recoveries are 31kpa, 48kpa respectively. The mass fluxes of membrane modules are recovered.