Membrane Science and Technology

Position：Home >> Abstract

Separation and purification of Poly(β-L-malic acid) in fermentation broth of Aureobasidium pullulans by a two-step ultrafiltration

Authors： Bi Yicheng, Wang Hao, Cheng Ruodong, Zhou Hua*, Wei Ping

Units： College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 211316, P. R. China.

KeyWords： Poly(β-)malic acid; two-step ultrafiltration; separation;

ClassificationCode：TQ028.8

year,volume(issue):pagination： 2015, 35(1):97-102

Abstract：

In this study, a two-step ultrafiltration was applied to separate and concentrate PMLA from fermentation broth. Membranes with MWCO of 30 kDa and 1 kDa were feasible for separating PMLA from Pullulan and concentration, respectively. The best operational conditions of 30kDa membrane were as follows: transmembrane pressure 0.075MPa, dilution factor of 4, volume reduction ratio of 8, times of dilution diafiltration 1. The concentration factor of 1 kDa membrane was 4. At the optimal conditions, the purity of PMLA increased to 82.3%, and the recovery rate was 57.56%.

Funds：

AuthorIntro：

毕艺成（1990-），女，安徽省怀宁人，硕士研究生，主要研究方向为生物高分子的提取与应用。

Reference：

[1] 李睿颖, 许勤虎, 刘晓鸥等. 聚苹果酸及其衍生物的研究与发展[J]. 食品工程，2009，(2)： 6～9.
[2] 程若东, 王浩, 周华等. 出芽短梗霉积累聚苹果酸途径及调控研究[J]. 化工学报，2012，(63)：3639～3644.
[3] 王浩, 郑谊丰, 程媛媛, 离子交换法从发酵液中提取聚苹果酸[J]. 离子交换与吸附，2011，27(3)：257～263.
[4] 乔长晟, 王凤荣, 蒋磊等. 一种酶法去除普鲁兰多糖提取获得β-聚苹果酸的方法. 中国. CN 101560528A. 2009年5月21日.
[5] 宋存江, 孙村民, 马晨燕等. 同时获得副产物普鲁兰β-聚苹果酸的制备方法. 中国. CN 101100687A. 2008年1月9日.
[6] K. Keller, T. Friedmann, X. Boxman, The bioseparation needs for tomorrow[J]. Trends Biotechnol. 19 (2001) 438–441.
[7] M.H.M. Isa,D.E. Coraglia, R.A, et al. Recovery and purification of surfactin from fermentation broth by a two-step ultrafiltration process[J]. Membr. Sci. 296 (2007) 51–57.
[8] H.L. Chen, Y.S. Chen, R.S. Juang, Separation of surfactin from fermentation broths by acid precipitation and two-stage dead-end ultrafiltration processes[J]. Membr. Sci. 299 (2007) 114–121.
[9] 周义卉, 高学玲, 张斌等. 超滤膜澄清分离速溶茶副产品中茶多酚的研究[J]. 膜科学与技术. 2012, 32(1), 102-105.
[10] 郑谊丰, 周华, 王丽燕等. 高效液相色谱法测定发酵液中聚苹果酸方法的建立[J]. 食品科技. 2010, 35(4), 243~246.
[11] 郑谊丰. 发酵液中聚苹果酸的分离提取[D]. 南京工业大学. 2010.
[12] 邓长江, 李长清, 朱希强等. 蒽酮-硫酸法测定出芽短梗霉发酵液残糖和总糖含量的研究 [J]. 食品与药品, 2006, 8(11): 54-56.

Service：

【Download】【Collect】

《膜科学与技术》编辑部 Address: Bluestar building, 19 east beisanhuan road, chaoyang district, Beijing; 100029 Postal code; Telephone：010-80492417/010-80485372; Fax：010-80485372 ; Email：mkxyjs@163.com