分别采用分光光度法和总有机碳（TOC）测定法测定超滤膜（大切割分子量和小切割分子量）对六种标示物（牛血清蛋白，鸡蛋白，α—糜蛋白，PEG20000，PEG10000，PEG6000）的截留率，以比较两种分析方法对膜表征的影响。结果表明：分光光度法和TOC法截留率测定结果较吻合。从数据稳定性来看，两种方法能较一致的测定出超滤膜的切割截留分子量；但是当溶液的截留率较低时，滤出液成分较复杂，两种方法所得结果数据则存在一定差异。还指出了分光光度法存在蛋白泡沫问题，样液浓度选择、稀释问题以及PEG显色时间影响问题，并介绍了避免或减轻以上情况的措施。通过比较，发现TOC测定法快速，稳定，精确度高，环保等优点。

The comparison between the methods of ultraviolet Spectrophotometry and total organic carbon analysis to determine the rejection of the ultrafiltration membrane was made based on studies of the ultrafiltration performance of PVDF membrane and PES membrane in this paper. Through 10 series of parallel tests using 6 probe solutes (egg white albumin and bovine serum albumin,α-chymotrypsin and PEG2000,10000,6000), it could be indicated that: the results got from ultraviolet Spectrophotometry are mainly coincide with that of TOC analysis. Factor analysis was use to further discuss the influence of method choice. The problems of ultraviolet Spectrophotometry such as PEG coloration time were discussed. And the advantage including speediness, accuracy and environmental friendly will widen the application of the method of TOC analysis in the area of membrane’s appraisement.

[1] 华耀祖.超滤技术与应用[M]. 北京：化学工业出版社,2003.1-43,51-76,80-103,180-190,210-460.
[2] 超滤装置标准[s]. HJ/T271,2006.
[3] Chakrabarty B, Ghoshal A K, Purkait M K. Effect of molecular weight of PEG on membrane morphology and transport properties[J]. Journal of Membrane Science, 2008, 309: 209-221.
[4] Xu Zhen-Liang, Alsalhy Qusay F.. Effect of Polyethylene Glycol Molecular Weights andConcentrations on Polyethersulfone Hollow Fiber Ultrafiltration Membranes[J]. Journal of Applied Polymer Science, 2004, 91: 3398-3407.
[5] 卞晓锴，陆晓峰，施柳青. 蛋白质超滤过程及超滤膜的改性研究现状.膜科学与技术, 2001, 21: 46-51
[6] Mosqueda-Jimenez D B, Narbaitz R M, Matsuura T. Effects of preparation conditions on the surface modification and performance of polyethersulfone ultrafiltration membranes[J]. Journal of Applied Polymer Science, 2006, 99: 2978-2988.
 [7] Mosqueda-Jimenez D B, Narbaitz R M, Matsuura T, et al.. Influence of processing conditions on the properties of ultrafiltration membranes[J]. Journal of Membrane Science, 2004, 231: 209-224.
[8] Hwang J R, Koo S H, Kim J H, et al. Effects of casting solution composition on performance of poly(ether sulfone) membrane[J]. Journal of Applied Polymer Science, 1996, 60: 1343-1348
[9] Barzin J, Feng C, Khulbe K C, et al. Characterization of polyethersulfone hemodialysis membrane by ultrafiltration and atomic force microscopy[J]. Journal of Membrane Science, 2004, 237: 77-85.
[10] Miyano T, Matsuura T, et al. Effect of polyvinylpyrrolidone additive on the pore size and the pore size distribution of Polyethersulfone[J]. Chemical Engineering Communications, 1993, 119: 23-39.