采用聚偏氟乙烯（PVDF）中空纤维膜构建膜曝气生物膜反应器（MABR），研究不同曝气压力、pH、流速和C/N比对模拟生活污水处理效果的影响，并分析PVDF中空膜表面微生物的Alpha多样性、物种群落及群落组成。结果表明，构建的MABR可有效去除模拟生活污水中的化学耗氧量（COD）、氨氮（NH4+-N）和总氮（TN），在曝气压力0.6bar、水力停留时间12.0h、pH8、C/N比5、循环流速90mL/min下，MABR对模拟生活污水的处理效果达到最佳，COD、NH4+-N及TN的去除率分别为95.4%、79.6%和71.8%。此时，反应器生物膜微生物具有较高的群落丰富度和多样性，Chao和Shannon指数分别为456.3和2.67，表明MABR具有较好的生活污水处理性能和潜在应用价值。

 In this study, membrane aeration bioreactor (MABR) was constructed using polyvinylidene fluoride (PVDF) hollow fiber membrane module and was employed to treat synthetic domestic sewage under different aeration pressure, pH, circulating flow and C/N ratio. The microorganisms of MABR were analyzed in terms of Alpha diversity, species community richness and community composition. It was found that the constructed MABR could effectively remove COD, ammonia nitrogen and total nitrogen from domestic sewage. Under the conditions of aeration pressure=0.6 bar, pH=8.0, circulating flow=90 mL/min and C/N=5.0, the constructed MABR attained its optimal efficiency, showing COD, ammonia nitrogen (NH4+-N) and total nitrogen (TN) removals of 95.4%, 79.6% and 71.8%, respectively. The results of microbial analysis revealed that the biofilm of MABR under optimum conditions possessed higher microbial community richness and diversity, and the main microbials of biofilm were Saccharimonadia and Gammaproteobacteria on Class level. The constructed MABR exhibits good performance in treatment of domestic sewage and potential value in practical application.

[1] 王芳, 徐楠. 磷酸铵镁法处理高浓度氨氮废水的应用研究[J]. 苏州科技大学学报:自然科学版, 2019(3):46-49.
[2] 张鹏, 刘兴誉, 贾媛媛. 关于含氨氮废水处理技术研究进展[J]. 山东工业技术, 2019, 281(03): 41-52.
[3] 陈文华, 潘超群, 厉雄峰, 等. MABR技术在农村生活污水处理上的应用[J]. 水处理技术, 2019, 45(5): 126-134.
[4] RittmannBE. Aerobic biological treatment. Water treatment processes [J]. Environ Sci Technol, 1987, 21(2):128-136.
[5] 宫志杰. MABR用于上海城市河道水生态修复的初步研究[J]. 科学技术创新, 2019(32): 109-111.
[6] 侯飞飞.MABR去除高盐废水中COD和氨氮的基础研究[D]. 天津: 天津大学,  2013.
[7] 李慧星, 杜风光, 薛刚. 高通量测序研究酒精废水治理中厌氧活性污泥的微生物菌群[J]. 环境科学学报, 2016, 36(11):4112-4119.
[8] Pierre C, Bersillon J L, Huyard A. Bubble-free aeration using membranes: mass transfer analysis [J]. J Membr Sci, 1989, 47(1-2):91-106.
[9] Syron E, Casey E. Membrane-Aerated Biofilms for High Rate Biotreatment: Performance Appraisal, Engineering Principles, Scale-up, and Development Requirements [J]. Environ Sci Technol, 2008, 42(6): 1833-1844.
[10] 刘强, 杨凤林, 张兴文, 等. 碳管膜曝气膜生物反应器处理高浓度氨氮污水的研究[J]. 水处理技术, 2007, 33(1): 63-66.
[11] Casey E, Glennon B, Hamer G. Biofilm development in a membrane-aerated biofilm reactor: effect of intra-membrane oxygen pressure on performance [J]. Bioprocess Eng, 2000, 23(5): 457-465.
[12] 赵胜楠, 赵天楚, 高会杰. 硝化菌耐盐驯化及处理高含盐氨氮废水实验研究[J]. 水处理技术, 2017, 43(03): 56-58+63.
[13]吴丹,刘伟,张琦,等.高浓度氨氮废水处理方法研究进展[J]. 盐科学与化工, 2020, 49(4): 10-15.
[14] 李玫,李保安,兰美超,等.新型MABR生物脱氮过程研究进展[J]. 膜科学与技术, 2020, 40(1): 260-265.
[15] 何秋阳. 膜曝气生物膜反应器生物膜影响因素分析[J]. 四川环境, 2012(05): 102-105.
[16] 谷静丽, 洪军, 冯亮, 等. 膜曝气生物膜反应器处理餐饮废水的研究[J]. 水处理技术, 2014, 40(04): 114-117+125.
[17] Shinya M, Akihiko T, Satoshi T. Modeling of membrane-aerated biofilm: Effects of C/N ratio, biofilm thickness and surface loading of oxygen on feasibility of simultaneous nitrification and denitrification [J]. BiochemEng J, 2007, 37(1):98-107.
[18] Rogers M B, Firek B, Shi M, et al. Disruption of the microbiota across multiple body sites in critically ill children [J]. Microbiome, 2016, 4(1): 66-78.
[19] Ji P, Rhoads W J, Edwards M A, et al. Impact of water heater temperature setting and water use frequency on the building plumbing microbiome [J]. ISME J, 2017, 11(6): 1318-1331.